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Plant Biochemistry in the Era of Omics: Integrated Omics Approaches to Unravel the Genetic Basis of Plant Stress Tolerance

Authors: Zareen Sarfraz,Yusra Zarlashat,Alia Ambreen,Muhammad Mujahid,Muhammad Sajid Iqbal,Syeda Akash Fatima,Muhammad Shahid Iqbal,Rashid Iqbal,Sajid Fiaz
Journal: Plant Breeding
Publisher: Wiley
Publish date: 2025-3-19
ISSN: 0179-9541 DOI: 10.1111/pbr.13277
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On page 9, the paper introduces “QTL-seq” as a powerful technique but fails to distinguish it from standard QTL mapping or bulk segregant analysis with sequencing. Moreover, Table 2 lists many QTLs (e.g., Saltol, OsHKT1;1) that were discovered years before QTL-seq existed.

My question is :  How does the inclusion of historically known QTLs (discovered by linkage mapping or GWAS) support the paper’s focus on advanced omics? Does the author actually understand that QTL-seq is merely a specific NGS application, not a conceptual breakthrough? This misrepresentation suggests a shallow grasp of the field’s history.

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