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Therapeutic potential of Chromolaena odorata, Vernonia amygdalina, and Cymbopogon citratus against pathogenic Bacteria

Authors: Udensi Justina Ugochi,Anyanwu Charles Obinna,Emedoh Andrew Emeka,Anyanwu Emilia Oluchi,Danladi Makeri,Pius Theophilus,Ezera Agwu
Publisher: Springer Science and Business Media LLC
Publish date: 2025-1-2
ISSN: 2045-2322 DOI: 10.1038/s41598-024-84696-3
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The methodology for plant extraction and the agar well diffusion technique is well-documented, but the study lacks detailed chemical profiling of the active compounds in the extracts. Identifying and quantifying these bioactive constituents would not only corroborate the observed antimicrobial effects but also provide a basis for standardizing future applications. The absence of this data makes it difficult to attribute the efficacy specifically to known antimicrobial agents.

In Table 3, the inhibition zones at varying concentrations of plant extracts are presented, showing a concentration-dependent increase in antibacterial activity. However, the comparison with antibiotics in Tables 4–7 reveals a critical limitation: the antibiotic concentrations used (µg/disk) differ fundamentally from the mg/ml concentrations of plant extracts. This discrepancy in measurement units could skew the perceived efficacy of plant extracts versus antibiotics. The authors should address this issue to ensure a fair comparison.

Figures and tables are generally clear, but some lack visual contrast, which could hinder interpretation. For example, multiple strains’ overlapping zones in inhibition measurements need clearer differentiation. Including graphical representations of standard deviations or error bars would also enhance data transparency and reliability.

 

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