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Participant
1. Concern about Figure 1C.
Horizontal lines are visible in the bottom part of the left panel, and the top part of the right panel, with a sudden change in background coloring and patterns. They are positioned at the exact spot where interesting protein complexes could have been present.
See my concerns about Figure 1C here:
Viewing 5 replies - 1 through 5 (of 5 total)
Concern about Figure 5A. Two vertical cuts appear to be visible between the lanes 1 and 2, and lanes 4 and 5 of all three panels, suggesting these samples might not have been run on the same gel. In the Cdc34 delta panel, lane 1 appears very similar to lane 5.
See my concerns about Figure 5A here:
[the ScienceGuardians system has trouble uploading my 400 kb image so posting without the image]
Concern about Figure 6C-b. A vertical cut appears to be visible between the lanes 1 and 2 of both the top and bottom panel, suggesting these samples might not have been run on the same gel.
See my concerns about Figure 6C-b here:
[the ScienceGuardians system has trouble uploading my 450 kb image so posting without the image]
Viewing 5 replies - 1 through 5 (of 5 total)
Concern about Figure 4B and 4C.
In the top panel of Figure 4B-b, a cut appears to be visible between the left and right half of the blot image.
In the bottom panel of Figure 4B-b, a horizontal “patch” appears to be visible, with a lighter background than the rest of the blot.
In Figure 4C, vertical lines are visible.
See my concerns about Figure 4 here: