1. While host-related factors are acknowledged, the role of environmental stressors (e.g., temperature fluctuations, pH shifts, and nutrient availability) in biofilm development is not extensively explored. Are there studies linking specific stress responses to enhanced biofilm formation in S. suis?
2. The detection of S. suis biofilms in lung tissues provides strong evidence of in vivo biofilm development. However, are there quantitative data comparing biofilm biomass in different host tissues? Could biofilm formation be a tissue-specific adaptation, or does it vary across infection models?
3. The review discusses biofilm inhibition strategies but does not critically assess their translational feasibility. Have any anti-biofilm agents shown significant efficacy in animal models? Could combination therapies targeting both biofilm integrity and bacterial virulence be more effective?
