On Page 6, under Section 3.1: Molecular characterization of SsLEAP2, the authors state:
“SsLEAP2 contains a signal peptide sequence (position 1–29), a prodomain (position 30–53) and a mature peptide (position 54–99) (Fig. 1), in which four cysteine residues forming three disulfide bonds (Cys70 – Cys81, Cys76 – Cys86).”
This is scientifically impossible.
Four cysteine residues can form a maximum of two disulfide bonds, not three.
The statement “four cysteine residues forming three disulfide bonds” is chemically incorrect and reflects a fundamental misunderstanding of protein biochemistry.