In the method part, they say they do the gastrointestinal digestion using “INFOGEST static in vitro simulation” ; this is a standard method, good.
But then, in the results for Degree of Hydrolysis (DH) in Figure 1b, they write about the intestinal digestion going for “120–240 min”. The standard INFOGEST protocol is 2 hours for stomach and 2 hours for intestine, total 4 hours. But here, their graph and text talk about intestinal phase lasting up to 240 min (4 hours) and even more? This is very confusing.
If they change the time so much from the standard protocol, how we can trust their results about digestibility? Maybe the longer time itself makes the protein look more digestible, not the ACP treatment? This is a big problem for the main conclusion of the paper.