The seasonal variation in transporter gene expression suggests nutrient mobilization, but was nutrient depletion in soil or tissue analyzed to confirm this? The lack of AMT_CL2 and NRT1/PTR_CL6 expression in April—despite available nutrients—raises questions about regulatory mechanisms.
B50 showed higher gene expression but no significant biomass gain. Could this indicate a compensatory response rather than improved nutrient availability? Also, was altered root distribution due to substrate compaction considered a confounding factor?
Root transporter gene expression was higher, but was it correlated with actual nutrient content in plant tissues? PHT_CL2’s elevated expression in BP25B25 suggests phosphorus retention—was microbial activity explored as a contributing factor?