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Impact of Different Amounts of Biochar as Growth Media on Macronutrient Transport Systems of Carrizo Citrange Rootstocks and Related Expression Analysis

Authors: Paola Caruso,Maria Patrizia Russo,Maria Allegra,Biagio Torrisi,Giancarlo Fascella,Giuseppina Las Casas,Concetta Licciardello,Marco Caruso,Alessandra Caruso,Filippo Ferlito
Publisher: MDPI AG
Publish date: 2025-1-6
ISSN: 2077-0472 DOI: 10.3390/agriculture15010113
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The seasonal variation in transporter gene expression suggests nutrient mobilization, but was nutrient depletion in soil or tissue analyzed to confirm this? The lack of AMT_CL2 and NRT1/PTR_CL6 expression in April—despite available nutrients—raises questions about regulatory mechanisms.

B50 showed higher gene expression but no significant biomass gain. Could this indicate a compensatory response rather than improved nutrient availability? Also, was altered root distribution due to substrate compaction considered a confounding factor?

Root transporter gene expression was higher, but was it correlated with actual nutrient content in plant tissues? PHT_CL2’s elevated expression in BP25B25 suggests phosphorus retention—was microbial activity explored as a contributing factor?

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