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Participant
Could you clarify the statistical treatment of the kinetic data, particularly the error margins and replicates? Were control reactions conducted to assess substrate background hydrolysis? Given the structural homology with Cps3D, has HS1.09 oligomerization been experimentally tested? Additionally, could you confirm whether enzyme assays at 25°C reflect physiological conditions, and if raw kinetic data and NMR spectra will be publicly available for reproducibility?