Concern about Figures 3A and 3B:
Red boxes and arrows: In the p-Akt (Ser473) panel of Figure 3A, the shHMGA1-1 and shHMGA1-2 bands look much more similar than expected, with some details that might have been removed by ‘smudging’ in the right band.
Blue boxes and arrows: In the p-Akt (Ser473) panel of Figure 3B, the pIRES-HMGA1.1 and pIRES-HMGA1.2 bands look much more similar than expected, with one of the lanes shown in mirror image.