The study presents compelling evidence for convergent promoters as co-regulatory elements rather than transcriptional interference sites. However, clarification is needed on whether the observed correlations (e.g., Spearman coefficients) imply causation, particularly in the absence of mechanistic validation. Were alternative methods, such as CRISPR-based perturbations or polymerase stalling assays, used to confirm these findings? Considering previous reports on transcription interference, how do the authors reconcile their findings with models suggesting polymerase collision effects?
