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Dibutyl phthalate disrupts [Ca2 ]i, reactive oxygen species, [pH]i, protein kinases and mitochondrial activity, impairing sperm function

Authors: Seung Hyun Park,Myung Chan Gye
Publisher: Elsevier BV
Publish date: 2025-5
ISSN: 1001-0742 DOI: 10.1016/j.jes.2024.03.015
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The study identifies elevated ROS levels in DBP-treated spermatozoa using the DCFH-DA assay. However, this method is sensitive to multiple reactive species beyond ROS, such as reactive nitrogen species (RNS), and lacks specificity for the individual types of ROS generated. Given the central role of ROS in the proposed mechanism of mitochondrial dysfunction and sperm impairment, this raises concerns about the accuracy of attributing observed effects solely to ROS. Could the authors clarify if complementary techniques (e.g., electron paramagnetic resonance spectroscopy or specific ROS scavengers) were employed to validate the type and source of reactive species?

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