It would be important to question why no comparison was made using other marker systems like SSRs or SNPs, which are more widely validated for population genetics and breeding applications. Without a direct comparative analysis or benchmarking against other marker types, it remains unclear whether the observed polymorphism and clustering patterns using SCoT are as robust or informative as those that might have been generated with more established markers. How do the authors justify the exclusive reliance on SCoT markers, and what are the limitations of this approach in terms of reproducibility, marker transferability, or linkage to functional traits relevant for breeding?
