Considering the known potential of ultrasound to induce structural unfolding or aggregation depending on amplitude and duration, how did the authors verify that the enhanced functional properties were not offset by possible structural degradation or oxidative damage to sensitive amino acid residues during sonication? Was any secondary structure (e.g., FTIR or CD) or oxidation marker analysis (e.g., carbonyl content) performed to confirm structural integrity and minimize misleading interpretations of “improved” functionality?
