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A combined recombinase polymerase amplification CRISPR/Cas12a assay for detection of Fusarium oxysporum f. sp. cubense tropical race 4

Authors: Megan Ceris Matthews,Jos van der Linden,Isabelle Robène,Samuel Rozsasi,Beatrix Coetzee,Manuela Campa,Johan Burger,Uzoma Nobel Akwuruoha,Ndubuisi Johnkennedy Madufor,Willem Perold,Umezuruike Linus Opara,Altus Viljoen,Diane Mostert
Publisher: Springer Science and Business Media LLC
Publish date: 2025-1-19
ISSN: 2045-2322 DOI: 10.1038/s41598-025-85633-8
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Can the authors explain how the RPA-Cas12a assay performs under diverse field conditions, such as varying soil types, humidity levels, or the presence of environmental contaminants? Additionally, have they validated its reliability and specificity in real-world agricultural settings to ensure its practical applicability beyond controlled laboratory environments?

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