All constructs (e.g., MS188∆NLS-K3M, K4M, K3K4M) are discussed as if they behave consistently, yet no expression or stability confirmation (e.g., Western blot or fluorescence intensity quantification) is provided. If mutations affect folding or stability, altered localization might reflect degradation or misfolding, not true loss of NLS function.
Despite the inclusion of observed cell counts (e.g., “n = 45” in merged confocal panels), the study does not provide any statistical analysis to support claims of differential localization between wild-type and mutant constructs.
