The study emphasizes the importance of cycloalkyl ring size and position for activity but does not address potential steric hindrance effects in detail. Could the authors clarify whether computational modeling or docking studies were used to assess how steric factors at C-4 and C-5 influence interactions with Leishmania biological targets? Additionally, while cytotoxicity was evaluated, can the authors elaborate on the rationale behind specific concentration thresholds used for selectivity index calculations?
