The study identifies significant effects on neurotransmitter levels and inflammatory markers, yet it does not address potential variability due to hormonal fluctuations in the female Wistar rats used. Could the authors clarify whether estrous cycle phases were monitored or controlled during the experiments, as these could influence the observed biochemical and behavioral responses? Additionally, the planarian model shows increased locomotor activity with the extract but lacks a detailed mechanism linking this to neurotransmitter pathways. Could more in-depth analysis of neurochemical changes in planarians strengthen the translational relevance of these findings?
