The findings of the article are promising; however, a few critical points require clarification to strengthen the study:
While anthocyanins isolated from Vitis coignetiae Pulliat are a mixture of anthocyanins, it remains unclear which specific compound(s) drive the observed effects. Could the authors provide further insights or discuss the contribution of individual anthocyanins?
The study evaluates anthocyanins in isolation without comparing their efficacy to conventional chemotherapeutic agents used in HCC treatment, such as sorafenib. Including a control group treated with a standard drug would contextualize anthocyanins’ therapeutic potential relative to established treatments.
The heterogeneity in tumor sizes within the anthocyanins-treated group warrants further analysis. Additionally, data on anthocyanin bioavailability in the in vivo system would help link observed effects to physiologically relevant concentrations.