While the study demonstrates the antifungal activity of alpha-mangostin, the mode of action remains unclear. Could the authors provide more information on whether alpha-mangostin affects fungal spore germination, membrane integrity, or other cellular processes? A mechanistic analysis would greatly strengthen the biological plausibility of the findings.
The in vivo study on host plants lacks essential details regarding experimental conditions such as plant age, treatment intervals, and environmental factors (e.g., humidity, temperature). These parameters are critical for reproducibility and assessing real-world efficacy. Could the authors clarify these experimental conditions to ensure transparency and allow for independent validation?